Work

Simple method for cryopreservation of an anaerobic rumen fungus using ethylene glycol and rumen fluid

Year: 1995

Type: article

Abstract: The cryopreservation of an anaerobic rumen fungus, Piromyces communis OTS1, was examined at -84 degrees C using dimethyl sulfoxide, propylene glycol or ethylene glycol as cryoprotectants. Ethylene glycol was the most effective agent, combining high survival and low toxicity, followed by dimethyl sulfoxide and propylene glycol. Cell-free rumen fluid in the cryopreservation medium decreased the toxicity of the cryoprotectant agents and also had a protective action per se. A survival of 80% after 1 year storage was obtained when samples with an initial zoospore density of 5 x 10(4) zoospores/ml were equilibrated for 15 min in medium containing 0.64 M ethylene glycol and 5% cell-free rumen fluid, then frozen with dry ice and stored at -84 degrees C.

Source: FEMS Microbiology Letters

DOI: 10.1111/j.1574-6968.1995.tb07469.x

Authors: Masaru Sakurada, Yasuhiro Tsuzuki, Diego P Morgavi, Yoshifumi Tomita, Ryoji Onodera

Institution: University of Miyazaki

Language: English

Topic: Ruminant Nutrition and Digestive Physiology

Subfield: Animal Science and Zoology

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: bronze

Funders: Kagoshima University, Aberystwyth University, United Graduate School of Agricultural Sciences, Kagoshima University

OpenAlex ID: W2000318382