Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides1
Year: 1977
Type: article
Abstract: The modified triester approach has been further improved and refined to the synthesis of defined sequences of deoxyribo-oligonucleotides. Improvements include arylsulfonyltetrazoles as faster and milder condensing agents, benzenesulfonic acid to avoid depurination during deblocking of trityl protecting groups and improved chromatographic procedures for purification of triester intermediates and purification of the final product containing 3'-5' phosphodiester linkages.
Source: Nucleic Acids Research
DOI: 10.1093/nar/4.2.353
Authors: J. Stawinski, T. Hozumi, S.A Narang, C.P. Bahl, R. Wu
Institutions: National Research Council Canada, Cornell University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2000323760