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Protective Effects of Puerariae flos Against Ethanol-Induced Apoptosis on Human Neuroblastoma Cell Line SK-N-MC

Year: 2001

Type: article

Abstract: Puerariaeflos (PF) is an oriental medical herb for alcohol abuse. To investigate whether PF possesses protective effects against ethanol (EtOH)-induced cytotoxicity in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric analysis, DNA fragmentation assay, and reverse transcription-polymerase chain reaction were performed on SK-N-MC human neuroblastoma cells. Cells treated with EtOH exhibited several apoptotic features, while those pre-treated with PF prior to EtOH exposure showed a decreased occurrence of apoptotic features. In addition, PF pre-treatment inhibited the EtOH-induced increase in caspase-3 mRNA expression. These results suggest that PF may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.

Source: The Japanese Journal of Pharmacology

DOI: 10.1254/jjp.87.338

Authors: Mi-Hyeon Jang, Min-Chul Shin, Youn-Jung Kim, Joo-Ho Chung, Sung-Vin Yim, Ee-Hwa Kim, Yeunhee Kim, Chang-Ju Kim

Institutions: Kyung Hee University, Semyung University, Kangwon National University

Language: English

Topic: Medicinal Plants and Neuroprotection

Subfield: Complementary and alternative medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W2000395000

FWCI: 2.407

Cites: 16

Cited by: 21

Related to: 10