Work

Comprehensive Studies of Drug Resistance Mediated by Overexpression of Response Regulators of Two-Component Signal Transduction Systems in Escherichia coli

Year: 2003

Type: article

Abstract: In Escherichia coli, there are 32 open reading frames (ORFs) that are assumed to be response regulator genes of two-component signal transduction systems on the basis of sequence similarities. We cloned all of these 32 ORFs into a multicopy expression vector and investigated whether or not they confer drug resistance via control of drug resistance determinants. Fifteen of these ORFs, i.e., baeR, citB, cpxR, evgA, fimZ, kdpE, narL, narP, ompR, rcsB, rstA, torR, yedW, yehT, and dcuR, conferred increased single- or multidrug resistance. Two-thirds of them conferred deoxycholate resistance. Five of them, i.e., evgA, baeR, ompR, cpxR, and rcsB, modulated the expression of several drug exporter genes. The drug resistance mediated by evgA, baeR, and cpxR could be assigned to drug exporters by using drug exporter gene knockout strains.

Source: Journal of Bacteriology

DOI: 10.1128/jb.185.6.1851-1856.2003

Authors: Hidetada Hirakawa, Kunihiko Nishino, Takahiro Hirata, Akihito Yamaguchi

Institutions: The Institute of Scientific and Industrial Research, The University of Osaka, The University of Osaka, Japan Science and Technology Agency

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: Japan Society for the Promotion of Science

OpenAlex ID: W2000470168

FWCI: 2.714

Cites: 22

Cited by: 169

Related to: 9