Immunochemical quantification of plasma high density lipoprotein
Year: 1974
Type: article
Abstract: Quantitative analysis of serum high density lipoprotein (HDL) was carried out by the rocket immunoelectrophoresis using monovalent antiserum against HDL. The antisera were prepared in rabbits injecting each of two major subunit components of apoHDL. Subunit peptides of apoHDL were fractionated from normal human apoHDL by a gel-filtration through Sephadex G-200 suspended in 8M urea (Scanu et al., Biochemistry 8, 3306 (1967)). Two major subunit peptides, apoHDL (III) and apoHDL (IV) have been known immunochemically distinct. These two subunit peptides are equivalent to A-I and A-II peptide or apoLp-Gln-I and apoLp-Gln-II. Single line of precipitation was observed by rocket immunoelectrophoresis of serum samples on an agarose-gel plate containing eaah of anti-apoHDL (III) or anti-apoHDL (IV) serum. The linearity of the standard curve and the reproducibility of the determinations were good.HDL concentration in normal human subjects at age 18-22 years old revealed 106±18 and 121±8 units for male and female, respectively (100 units for reference serum). Concentration of HDL in various kinds of liver diseases were 40±30 units for acute hepatitis (7 patients), 92±20 units for chronic hepatitis inactive form (18 patients), 85±21 units for chronic hepatitis active form (8 patients), 80±31 units for cirrhosis (25 patients) and 50±33 units for hepatoma (8 patients). All of them decreased significantly from normal controls (109±19 units for 24 subjects). No significant differences were observed in the results of HDL determination whether measured by anti-apoHDL (III) or anti-apoHDL (IV) serum.
Source: SEIBUTSU BUTSURI KAGAKU
DOI: 10.2198/sbk.19.151
Institutions: Kyushu University, Kyushu University Hospital
Language: French
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2000725939