Work

Isolation and partial characterization of SSI-like protease inhibitors from Streptomyces

Year: 1992

Type: article

Abstract: We attempted to screen a series of Streptomyces subtilisin inhibitor-like (SIL) proteins among several Streptomyces strains by using a highly sensitive assay system established by us. Of six randomly tested strains, four were found to produce SIL inhibitors as their major secreted proteins, suggesting that they might be distributed in a high frequency among this genus. Three inhibitors exhibited inhibition of both subtilisin BPN' and trypsin. Comparison of the amino terminal sequences of these isolated proteins with those of other reported SIL inhibitors revealed that the beta 1- and beta 2-sheets in SSI were highly conserved.

Source: FEMS Microbiology Letters

DOI: 10.1016/0378-1097(92)90043-n

Authors: Seiichi Taguchi, Shuichi Kojima, Izumi Kumagai, Hiroshi Ogawara, Kin-ichiro Miura, Haruo Momose

Institutions: Tokyo University of Science, Gakushuin University, The University of Tokyo, Meiji (Japan)

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology (medical)

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

OpenAlex ID: W2000729321

FWCI: 3.973

Cites: 17

Cited by: 26

Related to: 10