Year: 2002
Type: article
Abstract: Recent studies have revealed that the platelet adhesive process under flow is tightly regulated by multiple ligand-receptor interactions. However, platelet morphological changes during this process, particularly its physiological relevance, remain unknown under blood flow conditions. Using epifluorescence and scanning electron microscopy, we evaluated the real-time changes in platelet morphology during a platelet adhesive process on a von Willebrand factor-coated surface under physiological high shear flow in a perfusion chamber. Here, we show that dynamic platelet shape changes occurring during distinct phases of the adhesive process are precisely regulated by "inside-out" and "outside-in" integrin signals and are also a key regulatory element in successful platelet thrombogenesis opposing rapid blood flow in vivo.
Authors: Mitsuhiro Kuwahara, Mitsuhiko Sugimoto, Shizuko Tsuji, Hideto Matsui, Tomohiro Mizuno, Shigeki Miyata, Akira Yoshioka
Institution: Nara Medical University
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Funder: Eli Lilly and Company
OpenAlex ID: W2000755827