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Neural stem cell specific fluorescent chemical probe binding to FABP7

Year: 2012

Type: article

Abstract: Fluorescent small molecules have become indispensable tools for biomedical research along with the rapidly developing optical imaging technology. We report here a neural stem cell specific boron-dipyrromethane (BODIPY) derivative compound of designation red 3 (CDr3), developed through a high throughput/content screening of in-house generated diversity oriented fluorescence library in stem cells at different developmental stages. This novel compound specifically detects living neural stem cells of both human and mouse origin. Furthermore, we identified its binding target by proteomic analysis as fatty acid binding protein 7 (FABP7), also known as brain lipid binding protein) which is highly expressed in neural stem cells and localized in the cytoplasm. CDr3 will be a valuable chemical tool in the study and applications of neural stem cells.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.1200817109

Authors: Seong-Wook Yun, Cheryl Leong, Duanting Zhai, Yee Ling Tan, Linda Lim, Xuezhi Bi, Jae-Jung Lee, Han Jo Kim, Nam-Young Kang, Shin Hui Ng, Lawrence W. Stanton, Young-Tae Chang

Institutions: Agency for Science, Technology and Research, Singapore Bioimaging Consortium, National University of Singapore, Life Sciences Institute, Genome Institute of Singapore

Language: English

Topic: Molecular Sensors and Ion Detection

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2000888419

FWCI: 2.655

Cites: 23

Cited by: 82

Related to: 10