Analysis of Seawaters for the Recovery of Culturable Vibrio parahaemolyticus and Some Other Vibrios
Year: 2001
Type: article
Abstract: We investigated the recovery of dormant and injured cells along with the normally culturable cells of Vibrio species with special emphasis on V. parahaemolyticus using both selective and non-selective media at moderate (20 C) and standard (37 C) culture temperatures from a bay water environment. Culture temperatures (20 or 37 C) did not affect the recovery of V. parahaemolyticus but did for other vibrios. We observed similar seasonality of V parahaemolyticus as in most other environmental studies. V. parahaemolyticus and other Vibrio species were recovered in higher numbers by a replica plating method compared to most probable number (MPN) and direct TCBS (thiosulfate citrate bile-salt sucrose) agar counts. Even with the replica plating method, however, vibrios number goes down to a minimum level and V. parahaemolyticus was undetectable during the cool temperature period of the year, although total bacterial cells and CFU on nutrient agar (with 2% NaCl) did not vary so much during the study period.
Source: Microbiology and Immunology
Institution: Okayama University
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
SDG: Life below water
Open Access status: bronze
OpenAlex ID: W2000988156