Work

A fourth ADP/ATP carrier isoform in man: identification, bacterial expression, functional characterization and tissue distribution

Year: 2004

Type: article

Abstract: The mitochondrial ADP/ATP carriers (AACs) catalyze the exchange of cytosolic ADP for matrix ATP. We have identified and characterized a novel member of the AAC subfamily of mitochondrial metabolite transport proteins, termed AAC4. The AAC4 gene maps to human chromosome 4q28.1, and its product AAC4 is 66-68% identical to human AAC 1-3 and is localized to mitochondria. AAC4 transcripts are exclusively present in liver, testis and brain unlike those of AAC 1-3. Consistent with its belonging to the AAC subfamily, upon heterologous expression and reconstitution into liposomes AAC4 exchanges ADP for ATP by an electrogenic antiport mechanism with high specificity and high sensitivity to carboxyatractyloside and bongkrekic acid.

Source: FEBS Letters

DOI: 10.1016/j.febslet.2004.12.034

Authors: Vincenza Dolce, Pasquale Scarcia, Domenico Iacopetta, Ferdinando Palmieri

Institutions: University of Calabria, University of Bari Aldo Moro, Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies

Language: English

Topic: ATP Synthase and ATPases Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Ministero dell’Istruzione, dell’Università e della Ricerca