Work

Simultaneous Measurement of Local Glucose Utilization and Blood Flow in the Rat Brain: An Autoradiographic Method Using Two Tracers Labeled with Carbon-14

Year: 1983

Type: article

Abstract: A simplified technique that uses two radionuclide tracers has been devised to measure local cerebral glucose utilization (ICGU) and local cerebral blood flow (ICBF) in the same rat. The method employs [14C]-2-deoxyglucose and [14C]iodoantipyrine to produce an autoradiogram before and another after extraction into chloroform of the [14C]iodoantipyrine from the brain sections. The chloroform-extracted autoradiogram yields ICGU, and the difference in tissue carbon-14 concentration between the two autoradiograms permits calculation of ICBF. The double-isotope technique provides values of ICGU and ICBF that are statistically indistinguishable from those derived from conventional single-isotope methods.

Source: Journal of Cerebral Blood Flow & Metabolism

DOI: 10.1038/jcbfm.1983.7

Authors: Thomas W. Furlow, Lynn E. Harrison

Institution: University of Alabama at Birmingham

Language: English

Topic: Medical Imaging Techniques and Applications

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Office of Naval Research