A New Screening Method for Antidiabetics Based on the Intravenous Glucose Tolerance Test in Rabbits
Year: 1961
Type: article
Abstract: Effect of some antidiabetics on the blood sugar curve after intravenous injection of glucose was investigated with the rabbits pre-treated with priscoline. Since an action of priscoline was found to make the curve type exponential, configuration of glucose tolerance curve could be determined by two constants, e-k and C in a formula yt=ae-kt+C (y, blood sugar level; t, time; a, initial blood sugar).Influences of antidiabetics, insulin, tolbutamide (N-toluyl-sulfonyl-N'-butylurea), metformin (Dimethylbiguanide) and sodium mesoxalate injected intravenously, were as follows:(1)Examinations after single administrationa) After administration of 0.05u./kg of regular insulin, e-k and C became both smaller than those for control. They were influenced in the similar way by subcutaneous injection of 0.1u./kg. b) After injection of 100mg/kg of tolbutamide, C value decreased significantly without change in e-k value. c) 50mg/kg of metformin gave an increasing effect on both of the two constants.(2) Examinations after repeated administrationsAfter the administrations of 100mg/kg of sodium mesoxalate during 5 or 10 days, e-k became smaller but Clarger.(3) There were not any changes in e-k and I values at ter single administration of 100mg/kg of the mesoxalate, or after the repeated administrations of 0.05u./kg of insulin, 100mg/kg of tolbutamide or 31mg/kg of the mesoxalate.Mechanism of these actions was briefly referred to, and a possibility was discussed to apply this glucose tolerance test to the pharmacological screening method for antidiabetics.
Source: Endocrinologia Japonica
Authors: SETSUYA TAKEUCHI, MASUO TOBE, TOSHIKO SASAKI
Institution: Nippon Medical School
Language: English
Subfield: Endocrinology, Diabetes and Metabolism
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2004235914