MbtH-Like Proteins as Integral Components of Bacterial Nonribosomal Peptide Synthetases
Year: 2010
Type: article
Abstract: The biosynthesis of many natural products of clinical interest involves large, multidomain enzymes called nonribosomal peptide synthetases (NRPSs). In bacteria, many of the gene clusters coding for NRPSs also code for a member of the MbtH-like protein superfamily, which are small proteins of unknown function. Using MbtH-like proteins from three separate NRPS systems, we show that these proteins copurify with the NRPSs and influence amino acid activation. As a consequence, MbtH-like proteins are integral components of NRPSs.
Source: Biochemistry
DOI: 10.1021/bi1012854
Authors: Elizabeth A. Felnagle, John J. Barkei, Hyunjun Park, Angela M. Podevels, Matthew D. McMahon, Donald W. Drott, Michael G. Thomas
Institution: University of Wisconsin–Madison
Language: English
Subfield: Applied Microbiology and Biotechnology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2004263583