Testing by Sysmex UF-100 flow cytometer and with bacterial culture in a diagnostic laboratory: a comparison
Year: 2006
Type: article
Abstract: A large proportion of the samples tested in routine diagnostic microbiology laboratory are urine samples. The gold standard is bacterial culture, but a high proportion of samples cultured are negative. Unnecessary testing can be reduced and an improved service provided by an effective screening test. The Sysmex UF-100 flow cytometer has been developed to count cells and casts accurately in urine samples. Its performance in a screening test was compared with bacterial culture by using 1005 consecutive urine samples, and cut-off criteria were established. Cut-off values of 3000 bacteria/microl and 111 WBC/microl provided the best discrimination. Of 1005 samples, 606 (60%) would be cultured. Sixteen samples that were not selected according to these criteria were culture positive. This was considered acceptable for our routine use. The use of a testing algorithm incorporating the Sysmex UF-100 flow cytometer has improved the quality and efficiency of urine testing within the routine microbiology laboratory.
Source: Journal of Clinical Pathology
Authors: R Evans, M M Davidson, L R W Sim, A J Hay
Institution: Raigmore Hospital
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2004307365