Year: 1976
Type: article
Abstract: An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA, followed by Sarkosyl, after growth in the presence of carbenicillin. We also present a procedure for the isolation of the TI-plasmid on a large scale, that might be used for the mass isolation of other large plasmids which like the TI-plasmid, can not be cleared with earlier described procedures. The purity of the plasmid preparations was determined with DNA renaturation kinetics, which method has the advantage that the plasmid need not to be in the supercoiled or open circular form.
Source: Nucleic Acids Research
DOI: 10.1093/nar/3.2.449
Authors: A.M. Ledeboer, A.J.M. Krol, J.J.M. Dons, F. Spier, R.A. Schilperoort, I. Zaenen, N. van Larebeke, J. Schell
Institution: Leiden University
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2004454729