Work

Packaging recombinant DNA molecules into bacteriophage particles in vitro.

Year: 1977

Type: article

Abstract: Recombinant phage genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro. The process is efficient and nonselective and offers containment in initial stages of handling recombinant DNA. Ligase [poly(deoxyribonucleotide):poly-(deoxyribonucleotide) ligase (AMP-forming), EC 6.5.1.1] reaction products can recombine with endogenous phage DNA during packaging, but UV-irradiation eliminates the biological activity of the endogenous DNA.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.74.8.3259

Authors: B Hohn, K Murray

Language: English

Topic: Bacteriophages and microbial interactions

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2005458187

FWCI: 8.806

Cites: 28

Cited by: 336

Related to: 10