Work

Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase

Year: 2013

Type: article

Abstract: We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits.

Source: Transgenic Research

DOI: 10.1007/s11248-013-9764-x

Authors: Sanger Mouse Genetics Project, Edward Ryder, Brendan Doe, Diane Gleeson, Richard Houghton, Priya Dalvi, Evelyn Grau, Bishoy Habib, Evelina Miklejewska, Stuart Newman, Debarati Sethi, Caroline Sinclair, Sapna Vyas, Hannah Wardle-Jones, Joanna Bottomley, James Bussell, Antonella Galli, Jennifer Salisbury, Ramiro Ramirez-Solis

Institution: Wellcome Sanger Institute

Language: English

Topic: Animal Genetics and Reproduction

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,990

Funders: Wellcome Trust, National Institutes of Health, National Human Genome Research Institute, National Center for Research Resources

Awards: Consortium for large-scale production and cryopreservation of knockout mice, Consortium for Broad Based Disease Phenotyping of Knockout Mice, G4612446324, Consortium for large-scale production and cryopreservation of knockout mice

OpenAlex ID: W2007102240

FWCI: 0.987

Cites: 32

Cited by: 47

Related to: 10