Work

Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization

Year: 2013

Type: article

Abstract: Recently, we have disclosed that human KIAA1199 (hKIAA1199) is a hyaluronan (HA) binding protein implicated in HA depolymerization. Although a murine homologue (mKiaa1199) was previously cloned, no information about the function of the molecule was available. Here, we show that cells transfected with mKiaa1199 cDNA selectively catabolized HA via the clathrin-coated pit pathway. A glycosaminoglycan-binding assay demonstrated the specific binding of mKiaa1199 to HA. These results were similar to our observations with hKIAA1199, although slight differences were found in the peak sizes of the minimum degradates of HA. We conclude that like hKIAA1199, mKiaa1199 is a hyaladherin, leading to HA depolymerization.

Source: FEBS Open Bio

DOI: 10.1016/j.fob.2013.08.003

Authors: Hiroyuki Yoshida, Aya Nagaoka, Sachiko Nakamura, Yoshinori Sugiyama, Yasunori Okada, Shintaro Inoue

Institutions: Kanebo Cosmetics Inc. (Japan), Keio University

Language: English

Topic: Proteoglycans and glycosaminoglycans research

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,210