Year: 2007
Type: article
Abstract: The construction and performance of a novel enzyme based optical sensor for in situ continuous monitoring of glucose in biotechnological production processes is presented. Sensitive optical coatings are formed from inorganic–organic hybrid polymers (ORMOCER®sORMOCER®: Trademark of Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V. in Germany.) combined with a flurophore (ruthenium complex) and an enzyme, and applied to lenses, declad polymer optical fibre (POF) and polymer clad silica fibre (PCS). The enzyme, glucose oxidase, catalyzes oxidization of glucose to gluconic acid by depleting oxygen. Oxygen consumption is determined by measuring the fluorescence lifetime of metal organic ruthenium complexes which are quenched by oxygen. The coatings developed were designed to adhere to glass and polymer surfaces, to be compatible with enzymes and ruthenium complexes, and were demonstrated both as double- and single-layer structures. The sensor response to gaseous oxygen, dissolved oxygen and dissolved glucose was measured via fluorescence lifetime changes. A best detection limit of 0.5% (vol) has been determined for gaseous O 2 with selected ORMOCER® sensing layers. Glucose concentrations were measured to a detection limit of 0.1 mmol L −1 over a range up to 30 mmol L −1 . The sensor was usable for 30 days in a bioreactor. The opto-electronic instrumentation and performance in laboratory bioreactors and in an industrial reactor are evaluated.
Institutions: University of Manchester, Universidad Autónoma de Madrid, Czech Academy of Sciences, Institute of Chemical Process Fundamentals, École Centrale de Lyon, Centre National de la Recherche Scientifique, National Academy of Sciences of Ukraine, Laboratoire Ampère, Institute of Molecular Biology and Genetics, Czech Academy of Sciences, Teagasc - The Irish Agriculture and Food Development Authority, Fraunhofer Institute for Silicate Research, Safibra (Czechia)
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2007674008