Work

Crystallization and preliminary X-ray diffraction studies of ferredoxin reductase fromLeptospira interrogans

Year: 2006

Type: article

Abstract: Ferredoxin-NADP+ reductase (FNR) is an FAD-containing enzyme that catalyzes electron transfer between NADP(H) and ferredoxin. Here, results are reported of the recombinant expression, purification and crystallization of FNR from Leptospira interrogans, a parasitic bacterium of animals and humans. The L. interrogans FNR crystals belong to a primitive monoclinic space group and diffract to 2.4 angstroms resolution at a synchrotron source.

Source: Acta Crystallographica Section F Structural Biology and Crystallization Communications

DOI: 10.1107/s1744309106020136

Authors: Alessandro S. Nascimento, Thiago Ferrarezi, Daniela L. Catalano-Dupuy, Eduardo A. Ceccarelli, Igor Polikarpov

Institutions: Universidade de São Paulo, Instituto de Física de São Carlos, Consejo Nacional de Investigaciones Científicas y Técnicas, National University of Rosario, Instituto de Biología Molecular y Celular de Rosario

Language: English

Topic: Enzyme Structure and Function

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2008330293

FWCI: 0.174

Cites: 22

Cited by: 3

Related to: 10