Rational design and PCR-based synthesis of an artificialSchizophyllum communexylanase gene
Year: 1993
Type: article
Abstract: A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized and used in combination with flanking PCR primers to generate a 607 base pair gene which contained 31 unique locations for restriction enzyme cleavage. The amino acid sequence was tailored for expression in Escherichia coli by using only those codons found in highly expressed E. coli genes. The availability of the gene will facilitate analysis of the structure and function of this and other beta-(1,4) xylanases.
Source: Nucleic Acids Research
Authors: Roger W. Graham, Tom Atkinson, D.G. Kilburn, R.C. Miller, R.A.J Warren
Institution: University of British Columbia
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2008387200