Work

Characterization of a multilayer heparin coating for biomolecule presentation to human mesenchymal stem cell spheroids

Year: 2014

Type: article

Abstract: Mesenchymal stem cells therapies have the potential to treat many pathologies, however, controlling cell fate after implantation remains challenging. We have used a multilayer technology to graft a range of 5 μg/mL - 5 mg/mL heparin onto the surface of MSC aggregates. Heparin coating does not affect cell viability (seen through LIVE/DEAD staining), cell anti-inflammatory properties (seen through co-culture with activated monocytes)and facilitates sequestration by coated cells of a growth factor (TGF-β1) that remains bioactive. This system can maximize therapeutic potential of MSC-based treatments because the cell surface-loaded protein could both signal to the cells to influence transplanted cell fate and be released into the surrounding environment to help repair injured tissue.

Source: Biomaterials Science

DOI: 10.1039/c3bm60271k

Authors: J. Lei, L. T. McLane, J. E. Curtis, J. S. Temenoff

Institutions: Georgia Institute of Technology, Emory University, The Wallace H. Coulter Department of Biomedical Engineering

Language: English

Topic: Mesenchymal stem cell research

Subfield: Genetics

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green