Work

Isolation and Characterization of Polymorphic Microsatellite Markers from the Chinese Medicinal Herb Atractylodes macrocephala (Asteraceae)

Year: 2012

Type: article

Abstract: Atractylodes macrocephala Koidz. (Asteraceae) is an economically important Chinese medicinal herb. In this study, 15 polymorphic microsatellite markers were developed from A. macrocephala using the compound microsatellite marker technique. Levels of polymorphism within the 15 markers were assessed using 83 individuals from two wild and two cultivated populations in China. The number of alleles per locus ranged from 2 to 20, with an average of 9.9 alleles. Observed and expected heterozygosities ranged from 0.083 to 1.000 and from 0.097 to 0.938, respectively. These markers will be valuable for germplasm classiﬁcation and identiﬁcation, as well as for assessing the genetic diversity and spatial genetic structure among wild and cultivated populations of A. macrocephala.

Source: International Journal of Molecular Sciences

DOI: 10.3390/ijms131216046

Authors: Li Zheng, Zhong-Da Shao, Zong-Chao Wang, Cheng-Xin Fu

Institution: Zhejiang University

Language: English

Topic: Genetic diversity and population structure

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,499

Funders: Oklahoma State University, National Natural Science Foundation of China