Work

The activity in vitro of growth hormone in reducing the respiratory quotient of rat diaphragm

Year: 1959

Type: article

Abstract: The administration to the rat under suitable conditions of crude anterior-pituitary extracts (Fisher, Russell & Cori, 1936), of extracts rich in growth hormone (Greaves, Freiberg & Johns, 1940) or of purified growth hormone (Greenbaum, 1953) can bring about a reduction of respiratory quotient of the animal as a whole.In important notes Recant (1952a, b) stated that effects of this sort could be induced in vitro by certain pituitary extracts, in- cluding some preparations of growth hormone, with isolated rat diaphragm suspended in glucosecontaining buffer.We have undertaken the present investigation to ascertain whether this effect can be elicited with the purified preparations of growth hormone made in this Laboratory.EXPERIMENTAL Animal&.Male albino rats of inbred laboratory strain, weighing 100-150 g. and maintained on a stock diet (Short & Parkes, 1949) were used.For the experiments with diaphragm food was withdrawn overnight and the animals were killed by decapitation.

Source: Biochemical Journal

DOI: 10.1042/bj0710243

Authors: P. R. Dasgupta, F. G. Young

Institution: University of Cambridge

Language: English

Topic: Growth Hormone and Insulin-like Growth Factors

Subfield: Endocrinology, Diabetes and Metabolism

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Medical Research Council