Work

Molecular Identification of Malaysian Pineapple Cultivar based on Internal Transcribed Spacer Region

Year: 2012

Type: article

Abstract: Identification of pineapple cultivar solely based upon traditional method which only based on morphology. However, this approach could results in inaccurate cultivar identification and inconsistent. In this study, we conducted phenetic analysis of 9 Malaysian pineapple cultivars based on DNA sequences of the internal transcribed spacer (ITS) region to evaluate utility of the region as a barcode in identification of pineapple cultivars and to determine phenetic relationships among cultivar. Genomic DNA was directly extracted, and ITS region was amplified and sequenced. Phenetic analysis revealed that the pineapple cultivars used in this study were classified into three groups with high sequence similarity among them. This clearly showed that the DNA barcode from ITS region have good discrimination power to distinguish the pineapple cultivar. Since the tree distinctly separated into three group of cultivar, consensus sequences served as DNA barcode for Malaysian pineapple cultivar can be constructed.

Source: APCBEE Procedia

DOI: 10.1016/j.apcbee.2012.11.025

Authors: Topik Hidayat, Farah Izana Abdullah, Chandrika Kuppusamy, Azman Abdul Samad, Alina Wagiran

Institutions: Indonesia University of Education, University of Technology Malaysia

Language: English

Topic: Pineapple and bromelain studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

Funder: Universiti Teknologi Malaysia