Year: 2006
Type: article
Study design: Observational Study
Abstract: Human Bocavirus in Hospitalized Children, South AfricaTo the Editor: In recent years, several novel respiratory viruses have been identified.These include human metapneumovirus (HMPV) (1), severe acute respiratory syndromeassociated coronavirus (2), human coronavirus (HCoV) NL63 (3,4), HCoV HKU1 (5), and, most recently, human bocavirus (HBoV) (6).The latter belongs to the Parvoviridae family and is most closely related to bovine parvovirus and canine minute virus (CnMV), which are members of the genus Bocavirus (6).Parvovirus B19 and HBoV are the only 2 parvoviruses known to be pathogenic to humans, but the relevance of HBoV infection in the clinical setting is not known.In this retrospective study, 341 nasopharyngeal and bronchoalveolar lavage samples were taken from children (age 2 days-12 years) hospitalized with respiratory tract infections in 2004 in the Red Cross War Memorial Children's Hospital, Cape Town, South Africa.Samples were originally screened by using an indirect immunofluorescence assay (Light Diagnostics, Chemicon International, Temecula, CA, USA) for common respiratory viruses, including respiratory syncytial virus; influenza virus A and B; parainfluenza viruses 1, 2, and 3; adenovirus; and cytomegalovirus.Subsequently, HMPV and HCoV NL63 were detected by using reverse transcription-PCR (1,3).Samples were also screened for HBoV DNA.DNA was extracted by using the QIAamp DNA blood mini kit according to the manufacturer's instructions (Qiagen Inc., Valencia, CA, USA).PCR amplification of a region of the NP-1 gene and the 3′ portion of the VP1/2 capsid gene of HBoV was performed.Briefly, 10 µL
Source: Emerging infectious diseases
Authors: Heidi Smuts, Di Hardie
Institution: University of Cape Town
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W2010886142