Work

Cytotoxicity and Apoptosis Induction of Bovine Alpha-lactalbumin-oleic Acid Complex in Human Breast Cancer Cells

Year: 2015

Type: article

Abstract: Breast cancer is the mostly commonly diagnosed cancer in women worldwide. Using food-based approach to breast cancer chemoprevention has attracted a great deal of attention in recent years. In the present study, α-lactalbumin was purified from fresh bovine milk and the complex with oleic acid (BLA-OA Complex) was prepared by chromatographic method. The cytotoxic and apoptotic activities of BLA-OA complex on human ER-positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells was investigated by morphological observation, MTS assay, LDH release, DAPI staining, DNA ladder assay and cell cycle analysis. The results showed that BLA-OA complex inhibits cells growth and induces apoptosis in both human ER-positive MCF-7 and ER-negative MDA-MB-231 breast cancer cell lines. The findings of this study are positive for cow milk industry and human breast cancer chemoprevention.

Source: Food Science and Technology Research

DOI: 10.3136/fstr.21.103