Work

Enhanced Saccharification by Trichoderma reesei Expressing a ^|^beta;-Glucosidase from Magnaporthe oryzae

Year: 2012

Type: article

Abstract: A his7-tagged β-glucosidase from Magnaporthe oryzae (MoCel3A) was expressed under the control of a cellobiohydrolase I (cbh1) promoter in Trichoderma reesei. Accumulation of extracellular his7-tagged MoCel3A and glucose production were evaluated during growth on a variety of substrates. Enzyme preparations obtained from the recombinant T. reesei strain grown in the presence of cellulose produced more his7-tagged MoCel3A, exhibited greater hydrolytic activity towards pNPG and produced more glucose from PSC, cellulose, rice straw and laminarin than the wild type parent. These results indicate that expression of his7-tagged MoCel3A enhanced glucose production not only from β-1,4-glucan but also from β-1,3-glucan. More his7-tagged MoCel3A accumulated when the recombinant strain was grown in the presence of cellooligosaccharides with various degrees of polymerization. Interestingly, accumulation of his7-tagged MoCel3A was highly enhanced by growth on laminarioligosaccharides. An active enzyme preparation was obtained from the recombinant strain grown on rice straw, an inexpensive and renewable substrate.

Source: Journal of Applied Glycoscience

DOI: 10.5458/jag.jag.jag-2011_018

Authors: Machiko Takahashi, Masahiro Nakajima, Yuki Nakano, Takumi Takeda

Institution: Iwate Biotechnology Research Center

Language: English

Topic: Biofuel production and bioconversion

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: bronze

Funder: Japan Society for the Promotion of Science

Award: Engineering of a protein that enhances cellulose saccharification