Comparison of Classical Ion Exchange Amino Acid Analysis and o-Phthaldialdehyde Methods to Characterize Proteolysis by Lactobacillus bulgaricus
Year: 1989
Type: article
Abstract: Seven strains of Lactobacillus bulgaricus were assayed for proteolytic activity using o-phthaldialdehyde and classical ion exchange amino acid analysis.The strains were ranked in the same order of proteolysis by both methods.Linear regressions of total and individual amino acid concentrations, except for alanine and glycine, were correlated with o-phthaldialdehyde results (R 2 = .95).The mean coefficient of variation was 7.3% for amino acid analyses and 10.2% for o-phthaldialdehyde.The ophthaldialdehyde method was comparable to amino acid analysis for measuring gross proteolysis, but amino acid analysis provides additional information.
Source: Journal of Dairy Science
Institution: Utah State University
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2012345259