A Comparative Study of the Three Cucumber Cultivars Using Fluorescent Staining and Fluorescence In Situ Hybridization
Year: 2011
Type: article
Abstract: A molecular cytogenetic survey of Cucumis sativus L. revealed a chromosomal polymorphism among 2 European pickling cultivars of ‘Borszczagowski’ and ‘Monastyrski’, and one Japanese cultivar of ‘Zibai’ by fluorescent banding method with chromomycin A3 (CMA) and 4′,6-diamidino-2-phenylindole (DAPI), and fluorescence in situ hybridization (FISH) with the probes of 5S, 45S rDNA and telomere repeat sequences. Heterochromatic banding polymorphism was found in some chromosomes with CMA and DAPI staining, whereas no chromosomal polymorphism was found in FISH. Thus, the FISH allowed us to identify all of homologous chromosomes in each cucumber cultivars, while CMA and DAPI banding showed an easy detection of the polymorphism in cucumber chromosomes, even in close related cultivars between European pickling cultivars.
Source: CYTOLOGIA
Authors: Yoshikazu Hoshi, Kouhei Yagi, Masaki Matsuda, Hideyuki Matoba, Norikazu Tagashira, Wojciech Pląder, Stefan Malepszy, Katsuya Nagano, Atsumi Morikawa
Institutions: Tokai University, Nihon University, Hiroshima Jogakuin University
Language: English
Subfield: Genetics
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2013149061