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Evidence for communication between nerve growth factor and protein tyrosine phosphorylation

Year: 1990

Type: article

Abstract: Nerve growth factor (NGF) stimulation of PC12 cells activated two myelin basic protein (MBP) kinase activities greater than 10-fold within 5 min, which were resolved by chromatography on Mono Q. Each enzyme phosphorylated MBP on threonine and was inactivated by incubation with either CD45, a protein tyrosine phosphatase, or protein phosphatase 2A (PP2A), a serine/threonine phosphatase. The effects of CD45 and PP2A were prevented by vanadate and okadaic acid, respectively. Activation of the MBP-kinases provides a mechanism for communication between NGF and intracellular protein tyrosine phosphorylation.

Source: FEBS Letters

DOI: 10.1016/0014-5793(90)80386-w

Authors: Néstor Gómez, Nicholas K. Tonks, Christine Morrison, Tony Harmar, Philip Cohen

Institutions: University of Dundee, University of Washington, University of Edinburgh

Language: English

Topic: Nerve injury and regeneration

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2013403124

FWCI: 7.528

Cites: 19

Cited by: 103

Related to: 10