Work

Sub-diffraction resolution pump-probe microscopy with shot-noise limited sensitivity using laser diodes

Year: 2014

Type: article

Abstract: We demonstrate the use of intensity-modulated laser diodes to implement pump-probe microscopy and achieved sub-diffraction resolution imaging with shot-noise limited sensitivity with a scheme of balanced detection. This technique has several applications for various types of induced transmission change, including excited-state absorption, ground state absorption bleaching and stimulated emission. By using our technique, biological imaging of mouse T cells and the axons of neurons in the cerebral cortex was demonstrated.

Source: Optics Express

DOI: 10.1364/oe.22.009024

Authors: Jun Miyazaki, Hiromichi Tsurui, Akiko Hayashi-Takagi, Haruo Kasai, Takayoshi Kobayashi

Institutions: University of Electro-Communications, Juntendo University, Japan Science and Technology Agency

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,270

Funder: Japan Society for the Promotion of Science