Work

Development of Exon-Primed Intron-Crossing (EPIC) PCR primers for the malaria vector Anopheles pseudopunctipennis (Diptera: Culicidae)

Year: 2012

Type: article

Abstract: Using the Anopheles gambiae Giles genome as a template, we designed, screened and identified 14 novel Exon-Primed Intron-Crossing (EPIC) PCR primer pairs for Anopheles pseudopunctipennis Theobald 1901, a major vector of human Plasmodium sp. in South America. These primers were designed to target the conserved regions flanking consecutive exons of different genes and enabled the amplification of 17 loci of which nine were polymorphic. Polymorphisms at these loci ranged from two to four alleles. Intron length polymorphism analysis is a useful tool, which will allow the study of the population structure of this mosquito species, which remains poorly understood.

Source: Comptes Rendus Biologies

DOI: 10.1016/j.crvi.2012.05.002

Authors: Frédéric Lardeux, Claudia Aliaga, Rosenka Tejerina, Raùl Ursic-Bedoya

Institutions: Institut de Recherche pour le Développement, Institut de Recherche pour le Développement, Simon Fraser University

Language: English

Topic: Genetic diversity and population structure

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2014765192

FWCI: 0.587

Cites: 43

Cited by: 8

Related to: 10