Work

Combination of Baicalein and Amphotericin B Accelerates Candida albicans Apoptosis

Year: 2011

Type: article

Abstract: Candida albicans is the most important human fungal pathogen. Amphotericin (AmB) is a gold standard of antifungal treatment for fungi, but the severe side effect of this drug restricts its clinical application. In this study, the interaction of AmB and baicalein (BE) was investigated against thirty clinical isolates of C. albicans. Synergistic activities were determined using the checkerboard microdilution assay based on the fractional inhibitory concentration indices. Combination of BE and AmB accelerated C. albicans apoptosis accompanied with an increase of reactive oxygen species (ROS). Moreover, AmB increased the caspase activity and expression of the corresponding gene CaMCA1 in C. albicans. These effects were enhanced in the presence of BE. Deletion of CaMCA1 clearly attenuated AmB-induced apoptosis, indicating the involvement of CaMCA1-mediated caspase pathway in AmB-induced apoptosis and the synergistic action.

Source: Biological and Pharmaceutical Bulletin

DOI: 10.1248/bpb.34.214

Authors: ZiJin Fu, Hui Lu, ZhenYu Zhu, Lan Yan, YuanYing Jiang, YingYing Cao

Institution: Second Military Medical University

Language: English

Topic: Antifungal resistance and susceptibility

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Natural Science Foundation of China, Changhai Hospital of Shanghai