Work

Modification of the Methods for the Extraction of Carboxypeptidase and Proteinase Activities from Sorghum Malts

Year: 2003

Type: article

Abstract: The effects of two extractants on the activities of carboxypeptidase and proteinase enzymes from three sorghum varieties germinated for 3 and 5 days were studied. In all three varieties, cysteine hydrochloride (Cyst. HCl) proved a better extractant than 2-mercaptoethanol (2-ME) as shown by the increase in carboxypeptidase and proteinase activities with 5-day malts. A three-way analysis of variance (ANOVA) on the effect of germination time, extractant and variety on the carboxypeptidase activities of the three malts showed that all the factors plus their possible four-way interactions highly significantly (P < 0.001) affected the extractability/activity of the enzyme. However, while the three factors had a highly significant (P < 0.001) effect on the proteinase activity, one of their four-way interactions, specifically, extractant/variety, had no significant effect. This suggests that, unlike the carboxypeptidase enzyme, the effectiveness of the extractants in enhancing the extractability/activity of the proteinase enzyme in all the sorghum malt samples was amongst other factors, not variety-dependent.

Source: Journal of the Institute of Brewing

DOI: 10.1002/j.2050-0416.2003.tb00593.x

Authors: A. C. Ogbonna, S. K. C. Obi, B. N. Okolo

Institutions: University of Nigeria, University of Uyo

Language: English

Topic: Enzyme Production and Characterization

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $3,450