Work

The recombinant expression systems for structure determination of eukaryotic membrane proteins

Year: 2014

Type: review

Abstract: Eukaryotic membrane proteins, many of which are key players in various biological processes, constitute more than half of the drug targets and represent important candidates for structural studies. In contrast to their physiological significance, only very limited number of eukaryotic membrane protein structures have been obtained due to the technical challenges in the generation of recombinant proteins. In this review, we examine the major recombinant expression systems for eukaryotic membrane proteins and compare their relative advantages and disadvantages. We also attempted to summarize the recent technical strategies in the advancement of eukaryotic membrane protein purification and crystallization.

Source: Protein & Cell

DOI: 10.1007/s13238-014-0086-4

Authors: Yuan He, Kan Wang, Nieng Yan

Institutions: Tsinghua University, China-Japan Friendship Hospital

Language: English

Topic: Enzyme Structure and Function

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,700

Funders: Howard Hughes Medical Institute, National Natural Science Foundation of China, National Key Research and Development Program of China