Work

Broccoli Stunt, a New Disease in Broccoli Plants Associated with Three Distinct Phytoplasma Groups in Brazil

Year: 2013

Type: article

Abstract: Since 2007, a new disease in broccoli (Brassica oleracea var. italica Plenck) has been observed in the São Paulo state, Brazil. The characteristic symptoms of the disease are plant stunting, inflorescence malformation, reddening of the leaves and phloem necrosis. Nested polymerase chain reaction with P1/Tint and F2n/R2 primer pairs revealed the presence of phytoplasmas in diseased broccoli plants. Restriction fragment length polymorphism and phylogenetic analysis of the 16S rDNA gene showed that phytoplasmas belonging to 16SrI, III and XIII groups were associated with the plants. To the best of our knowledge, this is the first report of phytoplasmas in this Brassica species in Brazil, as well the first time phytoplasmas of 16SrIII and XIII groups have been associated with broccoli plants.

Source: Journal of Phytopathology

DOI: 10.1111/jph.12087

Authors: Bárbara Eckstein, Júlio César Barbosa, Patrícia Fabretti Kreyci, Maria Cristina Canale, Kátia Regiane Brunelli, Ivan Paulo Bedendo

Institutions: Embrapa, Embrapa Florestas, Universidade de São Paulo, Sakata (Brazil)

Language: English

Topic: Phytoplasmas and Hemiptera pathogens

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: green

Funders: Fundação de Amparo à Pesquisa do Estado de São Paulo, Science and Engineering Research Board