Work

Progesterone, but not β-estradiol, enhances Porphyromonas gingivalis lipopolysaccharide-induced vascular endothelial growth factor-A expression in human THP-1 monocytes

Year: 2013

Type: article

Abstract: Progesterone and estrogen levels are elevated during pregnancy and play a role in maternal immune responses. In addition, unbalanced metabolism of growth factors has been demonstrated in pregnancy tumors. Therefore, we aimed to investigate the effect of progesterone and β-estradiol on vascular endothelial growth factor-A (VEGF-A) and basic fibroblast growth factor (bFGF) messenger RNA (mRNA) expression in THP-1 monocytes in response to Porphyromonas gingivalis lipopolysaccharide (LPS). THP-1 monocytes were incubated with progesterone, β-estradiol, or LPS from Escherichia coli and P. gingivalis for up to 24 hours. The expression of VEGF-A and bFGF was investigated using conventional reverse transcription polymerase chain reaction (RT-PCR) and real-time RT-PCR. Cell growth was assessed using cell proliferation assay. We reported herein that progesterone, but not β-estradiol, increased VEGF-A mRNA expression in THP-1 monocytes. Significantly, progesterone enhanced VEGF-A mRNA expression in P. gingivalis LPS-treated monocytes in comparison with a treatment with P. gingivalis LPS alone. However, neither β-estradiol nor progesterone had any effect on bFGF production at mRNA levels. The enhancing effect of progesterone on VEGF-A mRNA expression may have a role in the pathogenesis of pyogenic granuloma in pregnant women.

Source: Journal of Dental Sciences

DOI: 10.1016/j.jds.2013.07.001

Authors: Paiboon Jitprasertwong, Niratcha Chaisomboon, Kusuma Jamdee

Institution: Naresuan University

Language: English

Topic: Oral microbiology and periodontitis research

Subfield: Periodontics

Field: Dentistry

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,600