Work

A new fabrication technique to form complex polymethylmethacrylate microchannel for bioseparation

Year: 2012

Type: article

Abstract: Recent studies show that reduction in cross-sectional area can be used to improve the concentration factor in microscale bioseparations. Due to simplicity in fabrication process, a step reduction in cross-sectional area is generally implemented in microchip to increase the concentration factor. But the sudden change in cross-sectional area can introduce significant band dispersion and distortion. This paper reports a new fabrication technique to form a gradual reduction in cross-sectional area in polymethylmethacrylate (PMMA) microchannel for both anionic and cationic isotachophoresis (ITP). The fabrication technique is based on hot embossing and surface modification assisted bonding method. Both one-dimensional and two-dimensional gradual reduction in cross-sectional area microchannels were formed on PMMA with high fidelity using proposed techniques. ITP experiments were conducted to separate and preconcentrate fluorescent proteins in these microchips. Thousand fold and ten thousand fold increase in concentrations were obtained when 10 × and 100 × gradual reduction in cross-sectional area microchannels were used for ITP.

Source: Biomicrofluidics

DOI: 10.1063/1.3683163

Authors: Talukder Z. Jubery, Mohammad R. Hossan, Danny R. Bottenus, Cornelius F. Ivory, Wenji Dong, Prashanta Dutta

Institution: Washington State University

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: bronze

Funders: Washington State University, National Institutes of Health, National Institute of General Medical Sciences

Award: Training in Biotechnology: Emphasis in Protein Chemistry