Specific capture of ABH blood group antigens of the red cell or body fluids by double antibody sandwich-ELISA.
Year: 1989
Type: article
Abstract: SAGISAKA, K., FLETCHER, S.M., KATSURA, S. and YOKOI, T. Specific Capture of ABH Blood Group Antigens of the Red Cell or Body Fluids by Double Antibody Sandwich-ELISA. Tohoku J. Exp. Med., 1989, 158 (3), 211-219-A double antibody sandwich-ELISA method for the detection of the ABH blood group of each constituent of mixed stains is described. Extracts from mixed stains were applied to microtitration plates coated with rabbit polyclonal antisera to red cells or body fluids. ABH antigens in body fluid stains which were captured by the polyclonal antibodies were detected by monoclonal anti-A and -B and enzyme-conjugated anti-mouse immunoglobulin. By this procedure, ABH antigens of only saliva, semen or red cells could be detected from mixed stains, butno ABH antigen capture activity was observed using anti-sweat, -milk, -vaginal secretion, -erythrocyte mem brane and -band 3 antibodies.
DOI: 10.1620/tjem.158.211
Institutions: Tohoku University, Home Office, Iwate Medical University
Language: English
Topic: Blood groups and transfusion
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2019522844