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Long-term two-photon neuroimaging with a photostable AIE luminogen

Year: 2015
Type: article
Abstract: In neuroscience, fluorescence labeled two-photon microscopy is a promising tool to visualize ex vivo and in vivo tissue morphology, and track dynamic neural activities. Specific and highly photostable fluorescent probes are required in this technology. However, most fluorescent proteins and organic fluorophores suffer from photobleaching, so they are not suitable for long-term imaging and observation. To overcome this problem, we utilize tetraphenylethene-triphenylphosphonium (TPE-TPP), which possesses aggregation-induced emission (AIE) and two-photon fluorescence characteristics, for neuroimaging. The unique AIE feature of TPE-TPP makes its nanoaggregates resistant to photobleaching, which is useful to track neural cells and brain-microglia for a long period of time. Two-photon fluorescence of TPE-TPP facilitates its application in deep in vivo neuroimaging, as demonstrated in the present paper.
DOI: 10.1364/boe.6.001477
Authors: Jun Qian, Zhenfeng Zhu, Chris Wai Tung Leung, Wang Xi, Liling Su, Guangdi Chen, Anjun Qin, Ben Zhong Tang, Sailing He
Institutions: State Key Laboratory of Modern Optical Instruments, Zhejiang University, Hong Kong University of Science and Technology, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, The Hong Kong University of Science and Technology (Guangzhou)
Language: English
Open Access status: gold
Estimated APC paid: $1,913
OpenAlex ID: W2022448522
FWCI: 1.041
Cites: 26
Cited by: 24
Related to: 10