Incorporation and maintenance of recombinant‐DNA plasmid vehicles pBR313 and pCR1 in plant protoplasts
Year: 1978
Type: article
Abstract: We have developed methods for inserting plasmid pBR3 13 of Escherichia coli and the large virulenceconferring plasmid pCKl35 of Agrobacterium tumefaciens into cowpea protoplasts [l-3].The uptake of pBR3 13 by barley protoplasts, which supports our findings, has been reported [4].Both small and large plasmids can be recovered from the nuclei of cowpea protoplasts exposed briefly to plasmid DNA under proper conditions [ 2,3].Although plasmid DNA replication and transcription, culminating in the synthesis of foreign gene products, have not been demonstrated in plant cells, the successful incorporation of plasmid DNA into protoplasts and their nuclei is the first step toward this demonstration.We now provide evidence for the stable maintenance of genecloning plasmids in protoplasts of carrots, cowpea, periwinkle and turnips, and provide conditions most conductive for plasmid uptake by these plant cells.2.
Source: FEBS Letters
Authors: S.M. Fernandez, P.F. Lurquin, C.I. Kado
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2031655804