Work

p185, an Immunodominant Epitope, Is an Autoantigen Mimotope

Year: 2011

Type: article

Abstract: An immunodominant peptide (p185(378-394)) derived from the c-erbB2 gene product, was recognized by an anti-DNA antibody, B3, and importantly by two classical DNA-binding proteins, Tgo polymerase and Pa-UDG. These reactivities were inhibited by DNA, confirming that the peptide mimicked DNA. BALB/c mice immunized with p185(378-394) developed significant titers of IgG anti-dsDNA antibodies. Screening of 39 human lupus sera revealed that 5% of these sera possessed reactivity toward p185(378-394). Representative mouse and human sera with anti-p185(378-394) reactivity bound intact p185, and this binding was inhibited by dsDNA. This is the first demonstration of a naturally occurring autoantigen mimotope. The present study identifies a potential antigenic stimulus that might trigger systemic lupus erythematosus in a subset of patients.

Source: Journal of Biological Chemistry

DOI: 10.1074/jbc.m111.224303

Authors: Sanjeev Kumar, John A. Hinks, Joseph Maman, Chelliah T. Ravirajan, Laurence H. Pearl, David A. Isenberg

Institutions: University College Hospital, University College London Hospitals NHS Foundation Trust, Institute of Cancer Research, University College London

Language: English

Topic: Systemic Lupus Erythematosus Research

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,430