Work

Protein phosphatase 2A is required for the initiation of chromosomal DNA replication

Year: 1998

Type: article

Abstract: Protein phosphatase 2A (PP2A) is an abundant, multifunctional serine/threonine-specific phosphatase that stimulates simian virus 40 DNA replication. The question as to whether chromosomal DNA replication also depends on PP2A was addressed by using a cell-free replication system derived from Xenopus laevis eggs. Immunodepletion of PP2A from Xenopus egg extract resulted in strong inhibition of DNA replication. PP2A was required for the initiation of replication but not for the elongation of previously engaged replication forks. Therefore, the initiation of chromosomal DNA replication depends not only on phosphorylation by protein kinases but also on dephosphorylation by PP2A.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.95.25.14693

Authors: Xiao-Hong Lin, Johannes Walter, Karl Scheidtmann, Kim Ohst, John Newport, Gernot Walter

Institutions: University of Bonn, University of California San Diego

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Cancer Institute, National Institute of General Medical Sciences

Awards: PROTEIN PHOSPHATASE 2A AND TUMOR ANTIGENS IN CELL GROWTH, G3693898079, MECHANISM OF DNA REPLICATION IN XENOPUS EXTRACTS, BIOCHEMICAL ANALYSIS OF NUCLEAR STRUCTURE