Year: 1987
Type: article
Abstract: Techniques for measuring methanol accumulated by resting cells of Methylosinus trichosporium in the narrow range of 70-80mM phosphate concentration have been standardized. The maximum amount of methanol, 6μmol•mg-1•hr-1, was accumulated at a cell density of 2mg•ml-1 (w/v) of cells in the early stationary phase at pH 6.5, temperature 35°C. The phosphate-dependent inhibition of methanol dehydrogenase was found to be uncompetitive and reversible. Further, 80mM phosphate inhibited methane monooxygenase and formate dehydrogenase by 16% and 20% respectively. A possible biochemical explanation of the role of phosphate inhibition is offered.
DOI: 10.2323/jgam.33.221
Authors: PERDEEP K. MEHTA, SAROJ MISHRA, TARUN K. GHOSE
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2040965892