Enantioseparation of Secnidazole by High-Performance Liquid Chromatography Using Amylose-Based Stationary Phase
Year: 2012
Type: article
Abstract: The secnidazole, a drug used in hepatic amebiasis, giardiasis and bacterial vaginosis, was enantioseparated on a semi-preparative scale by high performance liquid chromatography (HPLC), using chiral stationary phase (CSP) containing amylose tris (3, 5-dimethylphenyl) carbamate on silica gel (Chiralpak® AD). The experiments were carried out under normal phase conditions and the separation method exhibited very high selectivity and resolutions. The chromatographic parameters and the effects of column temperature on the retention time and selectivity were evaluated. Thermodynamic parameters were obtained from van't Hoff plots, and the elution order of the enantiomers was determined by circular dichorism. The enantiomers of secnidazole were separated under overloaded conditions with a good performance.
Source: Adsorption Science & Technology
Authors: Ana C. Nascimento, Rafael F. Perna, Marco A. Cremasco, Cesar C. Santana
Institution: Universidade Estadual de Campinas (UNICAMP)
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2041639628