Work

Overlapping Local and Long-Range RNA-RNA Interactions Modulate Dengue Virus Genome Cyclization and Replication

Year: 2015

Type: article

Abstract: The dengue virus genome is a dynamic molecule that adopts different conformations in the infected cell. Here, using RNA folding predictions, chemical probing analysis, RNA binding assays, and functional studies, we identified new cis-acting elements present in the capsid coding sequence that facilitate cyclization of the viral RNA by hybridization with a sequence involved in a local dumbbell structure at the viral 3' untranslated region (UTR). The identified interaction differentially enhances viral replication in mosquito and mammalian cells.

Source: Journal of Virology

DOI: 10.1128/jvi.02677-14

Authors: Luana de Borba, Sergio M. Villordo, Nestor G. Iglesias, Claudia V. Filomatori, Leopoldo G. Gebhard, Andrea V. Gamarnik

Institutions: Consejo Nacional de Investigaciones Científicas y Técnicas, Fundación Instituto Leloir

Language: English

Topic: Mosquito-borne diseases and control

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

Funder: National Institute of Allergy and Infectious Diseases

Award: MECHANISMS OF DENGUE VIRUS GENOME ENCAPSIDATION AND UNCOATING