Work

Gold nanorods for fluorescence lifetime imaging in biology

Year: 2010

Type: article

Abstract: Two-photon luminescence (TPL) from gold nanorods shows considerable potential in biological imaging. We study the imaging of gold nanorods in Madin-Darby canine kidney (MDCK) cells using fluorescence lifetime imaging microscopy (FLIM). FLIM provides images with better contrast and sensitivity than intensity imaging. The characteristic fluorescence lifetime of gold nanorods is found to be less than 100 ps, which can be used to distinguish gold nanorods from other fluorescent labels and endogenous fluorophores in lifetime imaging.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.3366646

Authors: Yinan Zhang, Jun Yu, David J. Birch, Yu Chen

Institutions: University of Strathclyde, Scottish Universities Physics Alliance

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: University of Strathclyde, Engineering and Physical Sciences Research Council

Award: Nanometrology for Molecular Science, Medicine and Manufacture