Work

Synthesis of Stable RNA in Stringent Escherichia coli Cells in the Absence of Charged Transfer RNA

Year: 1973

Type: article

Abstract: It has been possible to demonstrate the complete absence of either charged tRNA(Glu) or charged tRNA(Val) at 42 degrees by the use of two stringent strains of E. coli, one temperature-sensitive for glutamyl-tRNA synthetase and the other temperature-sensitive for valyl-tRNA synthetase. In both strains, stable RNA synthesis ceases, and guanosine tetraphosphate accumulates upon incubation at the nonpermissive temperature. Unique among a series of antibiotics tested, only tetracycline was able to stimulate stable RNA synthesis and to cause disappearance of the guanosine nucleotide. In this regard tetracycline and the "relaxed" gene product appear to be analogous.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.70.3.689

Authors: Samuel Kaplan, Alan G. Atherly, Anna Barrett

Institutions: University of Illinois Urbana-Champaign, University of Illinois System, Eli Lilly (United States), Iowa State University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2052394797

FWCI: 1.554

Cites: 22

Cited by: 34

Related to: 10