Work

A 17.6 kbp region located upstream of the rabbit WAP gene directs high level expression of a functional human protein variant in transgenic mouse milk

Year: 1992

Type: article

Abstract: We have investigated whether DNA regions present in the rabbit whey acidic protein (WAP) promoter/5' flanking sequence could potentially confer, in vivo, high level expression of reporter genes. Transgenic mice were generated expressing a variant of human alpha 1-antitrypsin, which has inhibitory activity against plasma kallikrein under the control of a 17.6 kbp DNA fragment located upstream of the rabbit WAP gene. Up to 10 mg/ml of active and correctly processed recombinant protein were detected in mouse milk, thus suggesting that the far upstream DNA sequences from the rabbit WAP gene might be useful for engineering efficient protein production in the mammary glands of transgenic animals.

Source: FEBS Letters

DOI: 10.1016/0014-5793(92)80683-8

Authors: Rainer Bischoff, Eric Degryse, Frédéric Perraud, Wilfried Dalemans, Dalila Ali-Hadji, Dominique Thépot, Eve Devinoy, Louis-Marie Houdebine, Andrea Pavirani

Institutions: Transgene (France), Institut National de la Recherche Agronomique

Language: English

Topic: Animal Genetics and Reproduction

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2052575270

FWCI: 2.702

Cites: 22

Cited by: 47

Related to: 10